trypsin solution (Thermo Fisher)

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Thermo Fisher trypsin solution
Trypsin Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypsin/Trypsin/pmc13094449-440-6-14
Average 99 stars, based on 1 article reviews

trypsin solution - by Bioz Stars, 2026-10

99/100 stars

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Staining:

Article Title: Noncanonical PI(4,5)P 2 coordinates lysosome positioning through cholesterol trafficking
Article Snippet: Cholesterol (C4951), filipin (F9765), methyl-beta-cyclodextrin (MCD) (C4555), 4-hydroxytamoxifen (SML1666), tamoxifen (10540–29-1), saponin (S4521), Triton X-100 (X-100), and corn oil (C8267) were obtained from Millipore Sigma. .. Paraformaldehyde (043368.9M), Lysotracker Red DND-99 (L7528), Hoechst nuclear stain (H3570), 4′,6-diamidino-2-phenylindole (DAPI; 62248), Goat anti-Mouse IgG (H + L) Secondary Antibody Alexa Fluor Plus 488 (A32723), Goat anti-Rabbit IgG (H + L) Secondary Antibody Alexa Fluor Plus 594 (A-11037), Goat anti-Rat IgG (H + L) Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (A-11006), Fumed Silica (S5505), Puromycin (A1113803), Geneticin (10131035), Trypsin 0.25% (25200056), Tet-system approved FBS (A4736401), and penicillin/streptomycin (15140122) were obtained from Thermo Fisher Scientific. .. Bafilomycin A1 (54645) and U18666A (68998) were obtained from Cell Signaling Technology.

Modification:

Article Title: Molecularly self-assembled nanoplatforms for targeted ROS-scavenging and neuroinflammation alleviation in acute ischemic stroke.
Article Snippet: Antibodies against GAPDH (60004-1-Ig), mTOR (66888-1-Ig), phosphorylated mTOR (p-mTOR, 67778-1-Ig), NeuN (66836-1-Ig), GFAP (16825-1-AP), CD86 (13395-1-AP), and CD206 (18704-1-AP) were provided by ProteinTech Group (Wuhan, China). .. IBA1 (A27316) was obtained from ABclonal Biotechnology Co., Ltd. Dulbecco's modified Eagle's medium (DMEM) and trypsin 0.25 % solution were obtained from Gibco (U.S.A.). .. Fetal bovine serum (FBS) was purchased from Xiaopeng Science & Technology Co., Ltd (Shanghai, China).

Article Title: An integrated multitarget screening strategy combining network pharmacology with cell membrane chromatography coupled to high-performance liquid chromatography for identifying candidate antidepressant compounds from Cyperus rotundus L.
Article Snippet: 31 Ethnopharmacological relevance: Cyperus rotundus L. (CR) is a traditional Chinese medicine 32 (TCM) herb that has been used for centuries to soothe the liver, regulate Qi, and relieve 33 emotional distress, with classical indications for “Yu syndrome” encompassing depressive 34 mood, chest distension, and dysphoria.. CR is incorporated into representative TCM formulas 35 targeting depression-related conditions, such as Yueju Pill, substantiating its 36 ethnopharmacological relevance in mood disorder management.. However, its complex 37 chemical composition presents considerable challenges for systematic bioactive compound 38 identification, and the specific antidepressant constituents of CR remain poorly characterized, 39 hindering the elucidation of its antidepressant material basis and the development of related 40 therapeutics.

Saline:

Incubation:

Article Title: Dual human milk oligosaccharide-fibre utilisation is a selection cue for the weaning gut microbiome.
Article Snippet: Four hundred nanograms of LysC protease (Wako) was added to each sample, followed by incubation for 4 hours at 37 °C with shaking at 750 RPM on a Thermomixer (Eppendorf). .. AR TI CL E IN P RE SS Samples were then diluted to a final tenfold dilution in digestion buffer, and 200 ng of trypsin (MS-grade, Thermo Scientific) was added to each sample before incubation for 18 hours and 30 minutes at 37 °C with shaking at 750 RPM. .. Samples were acidified by adding TFA to a final concentration of 1% (w/v), and the resulting peptides were desalted using a SOLA SPE plate (HRP 10 mg/2 mL, Thermo).

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